The inditification [i.e. identification] of the causal agent of the pear decline in Serbia | Identifikacija prouzrokovaca propadanja kruske u Srbiji
2006
Starovic, M., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Ivanovic, Z., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Aleksic, G., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Kuzmanovic, S., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Stojanovic, S., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Zivkovic, S., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia) | Gavrilovic, V., Institut za zastitu bilja i zivotnu sredinu, Beograd (Serbia)
The symptoms of pear decline are present in Serbia since 1970s. Thirty years later it was proved that the causal agent of this decline is a phytoplasma. This parasite is widespread in Europe and the USA as well as the countries of former Yugoslavia like Bosnia and Herzegovina and Croatia. The presence of the phytoplasmas was confirmed using electron microscopy and the molecular analysis of the pear samples from a number of localities in Serbia during the period of 2004-2007. The phytoplasma was observed on the ultrathin sections of the main leaf vascular bundle from the diseases pear tree. The shape, structure and size of 350-480 x 600-700 nm, are typical ones for the phytoplasmas. PCR analysis using the universal primer R16F2n/R16R2 as well as the specific primer fO1/rO1 also confirmed phytoplasmas as the causal agent of pear decline.
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