Production of polyphenolic compounds in shoot cultures of Hypericum species characteristic for the Balkan flora
2010
Danova, K., Sofia University St. Kliment Ohridski, Sofia (Bulgaria). Department of Plant Physiology
This work compares the polyphenolic compounds content in the in vitro schoot cultures of Hypericum rumeliacum, H. tetrapterum and in the hypericin non-producing H. calycinum, which are characteristic for the Balkan flora. In addition, the impact of different vitamin supplementation to culture medium on secondary metabolites is discussed. Though Gamborg vitamins supplementation improved growth parameters in vitro, it resulted in reduced polyphenolics levels in comparison with Murashige and Skoog vitamins. Preliminary experiments on cryopreservation of H. rumeliacum which were previously performed, gave low rates of plant survival. Therefore, as a part of a broader study for improving the approach for cryopresevation of H. rumeliacum, phenolics accumulation, total soluble sugars content as well as malondialdehyde and hydrogen peroxide content were studied during preculture treatment prior to dehydratation and liquid nitrogen immersion and results are discussed in this work.
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