Влияние липосом различного липидного состава на рост Mycobacterium tuberculosis H37Rv in vitro
2009
Sorokoumova, G.M., [email protected] | Petrova, E.E. | Zhogina, Yu.A. | Shvets, V.I., The M.V. Lomonosov Moscow State Academy of High Chemical Technology (Russian Federation) | Kalashnikova, T.Yu. | Selishcheva, A.A., Lomonosov Moscow State Univ. (Russian Federation) | Andreevskaya, S.M. | Smirnova, T.G. | Chernousova, L.N., Russian Academy of Medical Sciences, Moscow (Russian Federation)
Studied was the influence of liposomes of different lipid composition – phosphatilcholine, cardiolipine and glycosphinglipids on the growth and vitality of virulent strain Mycobacterium tuberculosis H37 Rv in vitro. The growth dynamics of culture in a liquid medium with liposomes was determined on a suspension of single mycobacterial cells at the same growth phase. The study involved liposomes from phosphatilcholine, cardiolipine and glycosphinglipids, as well as from a mixture of phosphatilcholine and glycosphinglipids in various ratios in concentrations of 50 to 750 micron/ml. The growth M. tuberculosis was fixed by PCR ad days 4, 9 and 14. As is shown, the effect of liposomes on the growth and vitality of M. tuberculosis H37 Rv in vitro depended on their lipid composition. Liposomes from phosphatilcholine had no effect in a broad range of comcentrations. Glycosphinglipids from swine brain inhibited the growth of M. tuberculosis H37 Rv. The addition to an incubation medium of phosphatilcholine liposomes comprising 25% of glycosphinglipids or liposome from glycosphinglipids alone has found true slowing down the growth of mycobacteria at days 9 and 14. Cardiolipine incorporated into a cellular membrane of mycobacteria in great concentration was capable of completely inhibiting the growth and vitality of M. tuberculosis H37 Rv. The inhibiting effect of cardiolipine is a signal function of this phospholipid in bacteria. It is supposed that the content of cardiolipine in the plasmatic membrane changes during propagation of M. tuberculosis thus adjusting the formation of domains which are a signal for transition of bacteria from active state to uncultivated state. The addition of exogenic cardiolipine at the starting moment of propogation of mycobacteria influence this process
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