Development and evaluation of monoclonal antibody-based enzyme linked immunosorbent assay for Borrelia garinii antigen detection in urine
2008
Gruntar, I., Ljubljana Univ. (Slovenia). Veterinary Fac. | Malovrh, T.
We developed antigen capture double sandwich ELISA (ACDS ELISA), based on borrelia flagellin-specific monoclonal antibody. Nested PCR analysis for borrelial DNA in the urine samples was used for comparison. Results obtained by ELISA and nested PCR analyses of urines from mice, experimentally infected with B. garinii by different routes of inoculation, confirmed the presence of borrelial antigens (antigenuria), while whole spirochetes or their DNA could not be detected or were not released into the urine (absence of spirocheturia). Such ELISA might become a good supplementary method in the diagnostics of Lyme disease, especially in seronegative subjects suspected to be infected with B. burgdorferi.
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