Rooting of in vitro-raised microcuttings of Magnolia Grandiflora, l. and M. x Soulangiana, soul.-bod. | Вкореняване на in vitro получени микрорезници от Magnolia Grandiflora, l. и Magnolia. x Soulangiana, soul.-bod.
2011
Gercheva, P., Fruit Growing Institute, Plovdiv (Bulgaria) | Nacheva, L., Fruit Growing Institute, Plovdiv (Bulgaria) | Ivanova, V., Agricultural University, Plovdiv (Bulgaria) | Ibrakhim, O., Fruit Growing Institute, Plovdiv (Bulgaria)
Magnolia grandiflora and M. x soulangiana are species with high aesthetic importance in addition to the medicinal and timber values of the former. Rooting in vitro is considered the main problem in the development of microculture of both species. The aim of the present study was to investigate the rooting ability of elongated in vitro shoots of both species. Rooting media supplemented with different concentrations of IBA - 0.0, 2.46, 4.92, 24.60 μM were used in the study. Shoots of M. grandiflora were further subjected to different treatments concerning: A. auxin level - 1.5 μM IBA, 1.5 μM IBA + 1.14 μM IAA and 1.5 μM IBA + 2.85 μM IAA; B. medium type - agar-based solid or perlite-based liquid medium and C. growing condition dark during the first 10 days or light 16-h photoperiod. In vitro rooting was not feasible in all cases. All rooted and nonrooted microshoots were assigned for ex vitro rooting and acclimatization in peat: perlite - 1:1. In 60 days a remarkable high rate of ex vitro rooting and survival was recorded in both species – up to 100% of the plants grown in vitro on agar-based medium with1.48µM IBA + 1.14µM IAA in under light conditions, followed by the same treatment with perlite-based medium 90%.
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