Differentiation of vaccine strain and field isolate of porcine pseudorabies virus by developed multiplex PCR | 多重PCR 方法快速鉴别猪伪狂犬疫苗毒和野毒
2009
Lin Fang, Chinese Academy of Agricultural Sciences, Lanzhou(China), Lanzhou Veterinary Research Institute | Yin Shuanghui, Chinese Academy of Agricultural Sciences, Lanzhou(China), Lanzhou Veterinary Research Institute | Shang Youjun, Chinese Academy of Agricultural Sciences, Lanzhou(China), Lanzhou Veterinary Research Institute
Chino. 根据Genbank 中PRV 的gB、gE、TK 基因序列设计并合成引物, 对样品中PRV DNA进行多重PCR扩增及反应条件优化, 得到与设计相符合的3 条特异性条带, 分别为427 bp( gB) 、298 bp( gE) 和208 bp( TK)。用这3 对引物对四种伪狂犬疫苗样品DNA 进行多次多重PCR 扩增, 其中1种疫苗得到与设计相符的1 条特异性条带, 其余为2条。该结果表明, 此检测方法敏感度较高, 适用于科学研究、临床诊断以及流行病学调查, 对根除伪狂犬病具有重要意义。
Mostrar más [+] Menos [-]Inglés. According to the sequence of PRV gB, gE , and TK gene published in GenBank, the primers were designed . Multiplex PCR ( multi-PCR) was developed and optimized to detect porcine pseudorbies virus , and the result revealed three specific bands at the expected size , respectively at 427 bp ( gB gene) , 298 bp ( gE gene) , and 208 bp ( TK gene) . Then four kinds of vaccine against pseudorabies virus were detected by the developed multi-PCR. One expected specific band appeared in one sample, while two bands in the others. The detection results showed that the multi-PCR has high sensitivity and should be applied to research, clinical diagnosis, and epidemiological investigation.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Institute of Agricultural Information, Chinese Academy of Agricultural Sciences