Transgenic sugar beet harbouring high-affinity nh_4~＋ transporter gene acquired by agrobacterium-mediated transformation | 农杆菌介导获得高亲和NH_4~＋转运因子转基因甜菜
2010
Huan Zhirong, Inner Mongolia Agricultural University, Hohhot(China) | Sun Yaqing, Inner Mongolia Agricultural University, Hohhot(China) | Li Guolong, Inner Mongolia Agricultural University, Hohhot(China)
Chino. 本研究利用农杆菌介导的方法将高亲和NH4＋转运因子融合基因转入甜菜子叶节外植体。遗传转化过程主要包括子叶节预培养、侵染转化、共培养、洗菌、抑菌培养、潮霉素筛选分化培养、抗性苗生根移栽。实验获得抗性苗140株,抗性芽分化率为8.14%,随机抽取24株抗性苗进行PCR检测,获得转基因植株10株。[著者文摘]
Mostrar más [+] Menos [-]Inglés. In this study, high-affinity NH4＋ transporter fusion gene was successfully transformed into the cotyledon node explants of sugar beet by using Agrobacterium-mediated transformation methord. Genetic transformation process includes cotyledon node pre-culture, infection transformation, co-culture, washed bacteria, bacteriostatic culture, differentiation culture of hygromycin selection, and resistance seedlings to root transplanting. As the experimental result, 140 resistant seedlings were obtained and its differentiation rate was 8.14%, 24 resistant seedlings were selected randomly to PCR detecte, and PCR assaying of plant DNA confirmed 10 transgenic plants.[著者文摘]
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