Micropropagation and morphogenesis of arracacha (Arracacia xanthorrhiza Bancroft)
2010
Sliva, S.,Ceska Zemedelska Univ., Prague (Czech Republic). Inst. Tropu a Subtropu | Viehmannova, I.,Ceska Zemedelska Univ., Prague (Czech Republic). Inst. Tropu a Subtropu | Vitamvas, J.,Ceska Zemedelska Univ., Prague (Czech Republic). Fakulta Lesnicka a Drevarska
The goal of our experiments was to establish an optimal protocol for micropropagation of arracacha. For multiplication phase MS medium, half-strength MS medium and MS medium supplemented with different concentrations of BAP and NAA was used. Indirect morphogenesis in callus cultures, induced from petioles, was tested on MS medium containing 2,4-D, KIN and on MS medium without plant growth regulators (PGR). For rooting of regenerated shoots MS medium without PGR and media containing NAA, 2,4-D, IAA and activated carbon were used. For multiplication of culture, MS medium supplemented with combinations of NAA and BAP was found to be the most effective (4.2+/-0.73 new shoots per explant within four weeks). On the contrary, no shoots were formed on half-strength MS medium. Higher frequency of callogenesis and shoot regeneration was achieved when explants were cultivated on the medium containing 2,4-D and subsequently transferred to MS medium without PGR. Low frequency of spontaneous rooting was observed in shoots on MS medium with/without activated carbon.
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