Molecular characterization of Campylobacter spp. isolated from poultry faeces and carcasses in Poland
2011
Wieczorek, K.,National Veterinary Research Inst., Pulawy (Poland). Dept. of Hygiene of Food of Animal Origin | Osek, J.,National Veterinary Research Inst., Pulawy (Poland). Dept. of Hygiene of Food of Animal Origin
The aim of the present study was to perform a molecular characterization of randomly selected Campylobacter jejuni and C. coli strains isolated from poultry faeces and carcasses in Poland. Several virulence gene markers were identified by polymerase chain reaction (PCR). Furthermore, genetic typing was also used by the macrorestriction profiling with pulsed-field gel electrophoresis (PFGE). The results of the present study showed that all analyzed isolates of C. jejuni (n = 24) and C. coli (n = 24) contained the flaA and cadF sequences. On the other hand, the virB11 gene was present only in 6 of 48 of the analyzed isolates, whereas most of the strains contained the cdt genes. Other virulence gene iam linked to Campylobacter invasiveness was present in 34 of 48 strains. The restriction analysis of the whole genome digested with SmaI produced three main clonal groups designed as I, II (with two subgroups IIa and IIb), and III obtained by the comparison of macrorestriction profiling patterns. The results showed a poor correlation between Campylobacter profiles generated by a clonal molecular technique and the presence of virulence markers. Therefore, PCR detection of Campylobacter virulence markers can be utilized as a simple and rapid tool to discriminate stains recovered from different sources, especially when used in conjunction with the PFGE profile analysis as a complex strategy.
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