Micropropagation of Gingo, ginkgo biloba L., plat by tissue culture
2010
Sarhan, A.Z. | Abou Dahab, A.E. | El-Bana, A.E. | Arafa, R.N.
The microcuttings were used as a source of explants at length (1.0 - 2.0 cm) which were removed from a tree then washed in soap water using septol soap for 30 min and rinsed with running tap water for 30 min. The explants were sterilized by Clorox [sodium hypochlorite (NaOC1)] at 20% for 20 min. Microcutting segments were cultured on Murashige and Skoog medium with various growth regulators, BA or Kin at different concentrations (0.0, 0.5, 1.0, 2.0 or 5.0 mg/1)the combination between 0.5 mg/l kin and different concentrations of NAA at (0.0, 1.0, 5.0 or 10 .0 mg/1), the combination between 0.5 mg/l IAA with different concentrations of BA at (2.0, 4.0, 8.0 or 10.0 mg/1) and NAA at (1.0, 2.0, 3.0, 4.0 or 5.0 mg/1). The cultures were incubated in the light and morphological development was recorded as the shoot length, petiole length, number and size of leaves. The maximum length of shoots was obtained when the microcutting segments were cultured on a medium supplemented with BA at 5.0 mg/l. However, shoots did not turn to plantlets, as they failed to root. BA considerably enhanced shoot proliferation from microcutting segments. The best growth recorded as the highest length of shoots (3.0 cm), the highest length of petiole (5.3 cm) and highest number of leaves (12.0 leaf/explant) was noticed on microcutting cultured on a medium supplemented with BA at 5.0 mg/l after two recultures.
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