Conversion of lactose to calcium lactobionate by some bacterial species
2010
Abd El-Hadi, A.A. | El-Minofi, H.A. | El-Beih, F.M. | Mohamed, N.M.H.
Bacterial conversion of lactose to calcium lactobionate was carried out by twelve strains of bacteria The strain Bacillus spp. E showed high lactose oxidizing activities, when cultivated at 30°C for 7 days in a medium ( pH 6) containing (g/100ml): lactose ,5; KH2P04,0.06 ; MgSO4.7H20, 0.025; corn steep liquor ,0.75 ml ; 1 drop of soybean oil ; 1.5m1 of 20% urea solution and CaC03,2.5 added during inoculation. The oxidizing activity was the highest where a relatively high lactose conversion (82%) was obtained at a lactose concentration of 50g/l . The activity remained almost stable between pH 5 and 6 and high conversion efficiency ( about 90%) was achieved at pH 0.6.
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