Development of molecular markers based on expressed sequenced tag linked to Fusarium head light resistance in wheat.
2009
Ghaffari, Mohammad Reza | Mardi, Mohsen | Laleh, Karimi Farsad
Fusarium head blight (FHB or scab) caused by Fusarium species can lead to tremendous loss of grain yield and quality in wheat (Triticum aestivum L.) as well as severe mycotoxin contamination. Detection of DNA markers linked to resistant gene(s) reduced the large number of backcross for transferring gene(s) from resistant parent to susceptible parent. The objective of this study was to increase the marker density using STS (sequence-tagged site) markers developed from wheat expressed sequence tags (ESTs) near Qfhs.ndsu -3BS. In this study, we used _Wangshuibai_ (resistant) /_Seri82_ (susceptible) and derived populations as plant materials. Out of 100 primer pairs designed from wheat ESTs, one EST was localized on chromosome 3BS. Based on our results, a QTL in the map interval of Xsts 3B -1 _ 138−Xgwm 493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16٪of the phenotypic variation. BLASTN analysis indicated that Xsts 3B -1 _ 138 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts 3B -1 _ 138 in _Wangshuibai_ at 96 h after inoculation was 2,6 times higher than _Seri82_. Our results underlined that EST-derived STS 3B- 138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
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