Genetic Linkage Mapping Using Interspecific Hybrid Population between Korean Wild Pear (Pyrus ussuriensis) and Japanese Pear (P. pyrifolia)
2010
Choi, J.K., Seoul National University, Seoul, Republic of Korea | Na, D.Y., Seoul National University, Seoul, Republic of Korea | Kim, D.I., Chungbuk National University, Cheongju, Republic of Korea | Shin, I.S., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea | Kim, M.Y., Seoul National University, Seoul, Republic of Korea | Lee, H.J., Seoul National University, Seoul, Republic of Korea
Genetic linkage maps for Asian pear (Pyrus spp.) were constructed based on RAPD, AFLP, and SSR markers using 94 seedlings of F₁ progenies from the cross between P. pyrifolia cv. Niitaka and P. ussuriensis cv. Suhyangri according to double pseudo-testcross mapping strategy. The map for 'Niitaka' consisted of 106 loci including 18 RAPDs, 85 AFLPs, and 3 SSRs on 19 linkage groups over a total length of 1,006 cM, while that for 'Suhyangri' contained 122 loci including 29 RAPDs, 88 AFLPs, and 3 SSRs on 19 linkage groups encompassing a genetic distance of 1,168 cM. These maps were partially aligned using two co-dominant SSR markers which showed segregating alleles in both parents. Although the linkage groups in the maps were not completely matched to 17 pear chromosomes, these linkage maps could be used as a basal framework for map-based cloning of valuable genes, analyzing quantitatively inherited traits, and tagging genes to increase the breeding efficiency in Asian pear trees.
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