Development of Immunoassay Kits for the Detection of Paenibacillus larvae Infection in Honeybee
2010
Kang, W.M., Jeju Special Self-Governing Province, Jeju, Republic of Korea | Yang, J.H., Equine Hospital of Busan Race Park, Korea Racing Authority, Busan, Republic of Korea | Jang, W.W., Jeju National University, Jeju, Republic of Korea | Son, S.W., Jeju National University, Jeju, Republic of Korea | Yoon, S.H., Jeju National University, Jeju, Republic of Korea | Hwang, K.K., Jeju National University, Jeju, Republic of Korea | Lim, Y.K., Jeju National University, Jeju, Republic of Korea
American foulbrood is very hamful bacterial disease caused by Paenibacillus larvae infection in honeybee, leads great damage to bee-farming and subsequently results in critical loss of productivity in many cases. To detect the infection of Paenibacillus larvae, the ELISA and the Immuno-chromatographic assay methods were developed using specific monoclonal antibodies against Paenibacillus larvae spore. Four of the monoclonal antibodies produced were identified to be IgG1 and one to be IgG2b. No cross-reactivity of the antibodies were detected with the 13 kinds of bacteria including Melissococcus pluton which is causative agent of European Foulbrood, indicating that the monoclonal antibodies were highly specific for Paenibacillus larvae. The detection limits of Paenibacillus larvae spore were determined to be 10∨5spore/ml, and the sensitivity and specificity were 96% and 100% respectively in ELISA. The detection limits of Paenibacillus larvae spore were determined to be 10∨7spore/ml, and the sensitivity and specificity were 92% and 100% respectively in immunochromatographic assay. These results suggested that the ELISA and immunochromatographic assay developed in this study could be applied in diagnosis of American foulbrood.
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