Melancortin receptor family evolution, focusing on ACTHR functional specificity. Doctoral thesis promotion to the degree of Doctor of biology, molecular biology
2011
Fridmanis, D., University of Latvia, Riga (Latvia). Faculty of Biology
Inglés. The aim of this research was to study the evolution of MCR family and identify functional dissimilarities determining structural regions of ACTHR. All MCRs were identified in fugu and zebrafish, no MCR were identified in tunicates or nematode. MCRs appeared and evolved particularly rapidly on an early stage of vertebrate evolution. Specialisation of ACTHR took place during the same period. To identify regions of ACTHR that determine its specificity chimeric receptors were created and tested for their membrane localization, ability to bind ligands and induce cAMP response. N-terminal part of ACTHR and 3rd and 4th TM are determinants of ACTHR intracellular trapping. 4th and 5th TM domains are involved in ACTH binding selectivity. An analysis method to quantify cell membrane localization of recombinant receptors was developed.
Mostrar más [+] Menos [-]Letón. Darba mērķis bija pētīt MCR saimes evolūciju un noteikt ACTHR funkcionālās atšķirības nosakošos strukturālos reģionus. Visi MCR tika atrasti fugu un zebrazivī, bet netika atrasti tunikātos un nematodē. ACR radās un attīstījās īpaši strauji ļoti agrā mugurkaulnieku attīstības posmā. ACTHR specializācija arī notika šajā pašā periodā. Lai noteiktu ACTHR specifiskumu nosakošos reģionus, tika izveidoti himeri receptori, kuriem noteica membrānas lokalizāciju, spēju saistīt ligandus un ierosināt cAMP atbildi. ACTHR N-gals un 3. un 4. TM ir receptora iekššūnas aizturi nosakošie reģioni. 4. un 5. TM ir iesaistīti ACTH saistīšanas specifiskuma nodrošināšanā. Tika izstrādāta analītiska metode rekombinanto receptoru membrānas lokalizācijas kvantificēšanai.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por Fundamental Library of Latvia University of Life Sciences and Technologies