Screening for potential anti-angiogenic property utilizing avian embryo chorioallantoic membranes (CAMs) 2. garlic (Allium sativum L.) oil
2011
Ramos, G.B. | Arguelles, C.L., De La Salle Univ., Taft Ave., Manila 2401 (Philippines). Biology Dept. | Herrera, A.A. | Ragasa, C.V., Philippines Univ. Diliman, Diliman, Quezon City 1101 (Philippines). Inst. of Biology
To examine the anti-angiogenic property of garlic (Allium sativum L.) oil, the blood vessel formation and branching complexity in the chorioallantoic membranes (CAMs) of avian embryos were measured quantitatively by histological microvessel point counting and macroscopic fractal dimension analysis. Surgical gelfoams, as matrix for the growing blood vessels, were implanted onto the CAMs. Seventy three-day old fertilized white leghorn eggs were divided into negative control (NC), which received phosphate buffered saline (PBS) no garlic oil, three positive control (PC) which received 20,50 and 100 ul PBS and three treatment groups (TG) which received 20, 50 and 100 ul garlic oil. The study lasted for 13 days during which samples were kept in constant 37 deg C. On the last day, the gelfoam implants with the surrounding CAMs were detached from the embryos and processed for light microscopy. A second batch of duck fertilized eggs was run in similar conditions. The CAMs were removed on the last day, mounted on water-absorbent white boards and photographed with macrolens for the fractal dimension analysis. Results showed that garlic oil significantly reduced the number of micro-vessels per area in the chick embryo CAMs in a dose-dependent manner, that is, the higher the dose, the lower the percent microvessel area incurred. A decreasing trend in blood vessel branching complexity was found in the fractal dimension analysis but not significantly different from the other groups. While there is a discrepancy in the results of the two assays, the potential anti-angiogenic property of garlic oil could not be disregarded. Possibly, an increase in the sample size could increase the power of the statistical test and may yield more conclusive results.
Mostrar más [+] Menos [-]Palabras clave de AGROVOC
Información bibliográfica
Este registro bibliográfico ha sido proporcionado por University of the Philippines at Los Baños