Pisum sativum Rhizobium interactions under different environmental stresses
2008
Naeem, F. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)) | Malik, K.A. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)) | Hafeez, F.Y. (COMSATS Inst. for Information Technology, Islamabad (Pakistan). Bioscience Dept.)
Effect of low temperature, salinity, nutrient level and photoperiod has been studied on 3 varieties of Pisum sativum var., P-48, meteor and AM-1 inoculated with Rhizobium strain PS-1. The plants were grown at 5, 10, 15, 20 and 25 degree C. The number of viable rhizobial cells was counted at each temperature by most probable number technique. The number of viable cells were constant at 5 and 10 degree C but increased between 15-30 degree C. Nodule formation was not observed at 5, 10 and 25 degree C at 15 degree C the nodules were less in number, whereas at 20 degree C best nodulation was observed. The pea plants were also subjected to different salinity levels i.e., 0, 1, 2, 3, 4, 5, 10 and 15dsm-1. High number of nodules was formed at 0 and 1dsm-1 whereas at 2 and 3dsm-1 the nodule number was reduced and yet at higher salinity levels no nodules were formed. Pea plants nodulated best at 12 hours photoperiod and 1/6th concentration of Hoagland's nutrient solutions. The mature
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