Effects of trypsinization on viability of equine chondrocytes in cell culture
2010
Sutradhar, B.C. (Chungbuk National Univ., Cheongju (Korea). Coll. of Veterinary Medicine) | Park, J. (Chungbuk National Univ., Cheongju (Korea). Coll. of Veterinary Medicine) | Hong, G. (Chungbuk National Univ., Cheongju (Korea). Coll. of Veterinary Medicine) | Choi, S.H. (Chungbuk National Univ., Cheongju (Korea). Coll. of Veterinary Medicine) | Kim, G. (Chungbuk National Univ., Cheongju (Korea). Coll. of Veterinary Medicine)
Trypsin is an essential reagent for routine cell culture work. In the cultivation of mammalian cells, it has been extensively used for cell isolation from tissues or cell dislodging in subculturing. It may damage the cell membrane in contact of cells during long trypsinization. However, there is no specific report on time-dependent effect of trypsinization on cells. In the present study, we investigated the time dependent effects of trypsinization on equine chondrocytes. Cell viability after trypsinization with 0.25% trypsin-EDTA for 5 to 60 minutes was quantified by trypan blue exclusion assay, propidium iodide-Hoechst double staining, flow cytometry analysis and XTT assay. The results showed that trypsin-EDTA decreased the proliferation of equine chondrocytes depending on the exposure time of trypsinization.
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