Optimization of microspore embryogenesis and plant regeneration protocols for Brassica napus
2011
Wan, G.L. (Zhejiang Univ., Hangzhou (China). Dept. of Agronomy) | Naeem, M.S. (Zhejiang Univ., Hangzhou (China). Dept. of Agronomy) | Geng, X.X. (Zhejiang Univ., Hangzhou (China). Dept. of Agronomy) | Xu, L. (Zhejiang Univ., Hangzhou (China). Dept. of Agronomy) | Li, B. (Zhejiang Univ., Hangzhou (China). Dept. of Agronomy) | Jilani, G. (PMAS Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Soil Science) | Zhou, W.J.
Composition of nutrient media, inflorescence length and in vitro growth duration of donor plant, as well as heat shock, activated charcoal and sucrose concentrations could have remarkable effects on embryogenesis. Contribution of these factors on embryogenesis and shoot regeneration in two genotypes of oilseed rape (Brassica napus L.) viz. ZS758 and ZS72 was evaluated in the present study. The highest normal embryo yield in both cultivars was obtained from the NLN induction medium supplemented with 0.25 mg mL-1 activated charcoal and 130 g L-1 sucrose, which was heat shocked in darkness at 30 degree C for 2 days. The largest shoot regeneration frequency from microspore-derived (MD) embryos of both cultivars was obtained from the embryos aged 4 weeks. Further, the maximum shoot regeneration frequency of both cultivars was achieved from MS differentiation medium supplemented with 0.3 mg L-1 BA, 2 mg L-1 NAA and 0.1 mg L-1 GA3.
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