Genetic variability analysis of a collection of old Portuguese bread wheat using ISSRs
2008
Carvalho, A.;Lima-Brito, J.;Guedes-Pinto, H., University of Trás-os-Montes and Alto Douro, Vila Real (Portugal). Centre of Genetics and Biotechnology | Maçãs, B., National Plant Breeding Station, Elvas (Portugal)
Forty-eight bread wheat cultivars of an old Portuguese collection using ISSR markers were analysed. We used 18 ISSR primers that amplified a total number of 245 ISSR loci, being 233 of them polymorphic. Considering the overall analysis, the percentage of ISSR polymorphism was lower, 40.6 per cent, since different primers amplified ISSR fragments with the same molecular weight in all the cultivars. After clustering analysis, we verified that most cultivars belonging to the same botanical variety were clustered in the same main group. However, an intra-variety ISSR polymorphism was also observed
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