In vitro propagation of Dianthus ciliatus ssp. dalmaticus and D. giganteus ssp. croaticus (Caryophyllaceae) from stem segment cultures
2010
Radojević, Lj., Institute for Biological Research Siniša Stanković, Belgrade (Serbia) | Ćalić-Dragosavac, D., Institute for Biological Research Siniša Stanković, Belgrade (Serbia) | Špirić, J., Institute for Biological Research Siniša Stanković, Belgrade (Serbia) | Stevanović, B., Faculty of Biology, Belgrade (Serbia). Institute of Botany and Botanical Garden Jevremovac | Stevanović, V., Faculty of Biology, Belgrade (Serbia). Institute of Botany and Botanical Garden Jevremovac
In conclusions: Micropropagation of two Dianthus species was achieved by stem segment culture; Formation of adventitious buds was possible via organogenesis for endemic species, D. ciliatus ssp. croaticus and D. giganteus ssp. croaticus; In white callus of D. ciliatus ssp. croaticus the formation of embryogenic like structure is probably possible via somatic embryogenesis; Also, earlier preliminary cytological studies showed that these two processes (organogenesis and somatic embryogenesis) may occur simultaneously in the calli of D. ciliatus (Radojevic et al. 2006, Radojevic et al. 2010), but organogenesis was more pronounced than somatic embryogenesis; In vitro plantlets of both endemic carnation species were planted in rocky garden of the Belgrade (Serbia) Botanical Garden Jevremovac where they even bloomed.
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