The introduction of a method for determination of organic matter digestibility in feeds into routine laboratory practice
2008
Pojić, M., Institute for Food Technology, Novi Sad (Serbia) | Palić, D., Institute for Food Technology, Novi Sad (Serbia) | Mastilović, J., Institute for Food Technology, Novi Sad (Serbia) | Janić- Hajnal, E., Institute for Food Technology, Novi Sad (Serbia)
The amount of available energy in feeds for ruminants can be described by organic matter digestibility (OMD), since the value of OMD is close to the corresponding digestibility of energy. In vivo trials with animals for determining OMD are expensive, time-consuming and not suited for routine analysis and there has been a constant search for alternative in vitro methods. The aim of this study was to introduce into routine laboratory practice an in vitro procedure for OMD estimation and to evaluate the possibility of its use for the development of near infrared spectroscopy (NIRS) calibration model for predicting OMD determined in vivo. Results of this study indicated existence of a certain bias, but it could not be considered as a significant spread since all obtained values were within the warning limits. Repeatability limit given to the in vitro method was fulfilled. Method showed such analytical characteristics that it can be utilized as the reference method for the development of NIRS prediction model.
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