UV-mutant analysis of Xanthomonas oryzae pv. oryzae strains and control efficacy against bacterial leaf blight of rice
2010
Amy Thein(Kasetsart University, Bangkok (Thailand). Faculty of Agriculture. Department of Plant Pathology) | Sutruedee Prathuangwong(Kasetsart University, Bangkok (Thailand). Faculty of Agriculture. Department of Plant Pathology) E-mail:[email protected]
27 strains of Xanthomonas oryzae pv. oryzae (Xoo) caused bacterial leaf blight of rice, from different rice growing regions of Thailand were collected and investigated for their antagonistic ability. Among them, 11 strains produced bacteriocin on selective medium, yeast peptone dextrose agar (YPDA). One out of eleven strains with highest production of bacteriocin, strain (XOO-19) was selected and attenuated by UV mutagenesis. The 700 strains of UV mutants were evaluated for disease initiation and hypersensitive response (HR) on susceptible rice cultivar, Jasmine 105 and tobacco plants respectively. From those mutants, 15 strains were completely lost pathogenicity and HR. Moreover, two mutants including strains M-407 and M-690 showed no production of cellulase and significant reduction in extracellular polysaccharides (EPS), amylase, and protease, the virulent factors of Xoo pathogenicity. Interestingly, production ability of bacteriocin and the motility of mutants and wild type were not significantly different. However, the bacteriocin produced by either wild type or mutant strains was tolerant to maximum temperature at 42 deg C. The control efficacy on bacterial leaf blight and reduction in population of bacteriocin sensitive strain were not significantly different between mutant and wild type suggesting that control efficacy of UV mutant or nonpathogenic strain may involve various mode of action than bacteriocin mechanism.
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