THE IMPORTANCE OF INOCULATION DOSE OF AVIAN H6N2 INFLUENZA A VIRUS ON VIRUS SHEDDING OF ANAS PLATHYRYNCHOS DUCKS AFTER INDUCED GENERALISED INFECTION
2012
Zarkov, I., Thracian University, Stara Zagora (Bulgaria)
A total of 32 30-day old Anas plathyrynchos ducks - 4 groups - No 1, No 2, No 3, No 4, each from 8 ducks - 6 infected and 2 control uninfected were used in this experiment. Some of the birds - 24 nambers were infected intravenously with LPAIV H6N2, isolated from wild ducks - Anas platyrhynchos. A control group was not infected. The infection was accomplished with different virus dose – group No 1 with 10 5.00 ELD50 /100 μL per bird, No 2 – with 10 4.00 ELD50 /100 μL per bird, No 3 - 10 3.00 ELD50 /100 μL per bird and No 4 - 10 2.00 ELD50 /100 μL per bird. In all groups the portion of infected birds, period of virus shedding were monitored. The virus reisolation showed that infection was established in ducks, infected with dose from 10 5.00 ELD50 /100 μL per bird to 10 3.00 ELD50 /100 μL per bird. More birds with virus reisolation - 100% were detected when inoculation was made with the higher virus dose of 10 5.00 ELD50 /100 μL per bird and 10 4.00 ELD50 /100 μL per bird. In dose 10 3.00 ELD50 /100 μL per bird virus reisolation were - 30 % while in dose 10 2.00 ELD50 /100 μL per bird virus reisolation is unsuccessful. The highest numbers of reisolates (50% of tested samples) and for the longest time - 21 days were at the highest dose of virus.
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