Study on the expression regulation and regulatory functions of miRNA-181b in the anterior pituitary of cattle | miRNA-181b ㄧ哄浣涓琛ㄨ揪瑙寰惰浣ㄧ绌
2011
Xu Cheng, Jilin University, Changchun (China), College of Animal Science and Veterinary Medicine | Xue Wenzhi, Jilin University, Changchun (China), College of Animal Science and Veterinary Medicine | Zhao Maoxin, Jilin University, Changchun (China), College of Animal Science and Veterinary Medicine
[Objective] The research aimed to discuss the differential expression quantity of miRNA-181b in mature (18-month-old) and immature (one- month-old) cattle's anterior pituitary and its regulation function. [Method] cDNA library of miRNA in mature (18-month-old) and immature (one-month-old) cattle's anterior pituitary were established. After Solexa high-throughput sequencing of miRNA in the cDNA library, miRNA in anterior pituitary of bulls was identified. miRNA-181b with differential expression were selected from the sequencing results. By real-time quantitative RT-PCR, the expression regulation of miRNA-181b in the anterior pituitary of Yanbian Cattle in different growth period was validated. And the target genes of miRNA-181b were forecast by using TargetScanS prediction software. [Result] The expression quantity of miRNA-181b had great difference in cattle's anterior pituitary different growth periods. The expression quantity of miRNA-181b in anterior pituitary of one-month-old cattle was 4.05 times as that in 18-month-old cattle. The binding of miRNA-181b with 838~844 bases in 3' untranslated region of FSH尾 gene was specific and the binding base sites were UGAAUGUA. [Conclusion] This experiment provided the theoretical basis for the transcription regulation research of FSH尾.
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