Genetic diversity analysis and fingerprinting of eggplant cultivars with SRAP markers | 应用SRAP标记对茄子品种进行遗传多样性分析与指纹图谱构建
2011
Li Huaizhi, Shanghai Jiao Tong University, Shanghai(China),School of Agricuhure and Biology
Chino. 摘 要：利用SRAP技术对36份茄子栽培品种进行了分子鉴定。结果表明：选用25对引物组合对其基因组DNA进行PCR扩增,共获得566条扩增带,其中多态性谱带102条,平均每个引物组合扩增出4.08条多态性条带;品种间‘望哈茄’与‘本溪早紫茄’遗传相似系数最大,为0.986,‘辐射1号’与‘沪紫长茄’间遗传相似系数为0.581。同时使用8对引物组合构建了36个品种的指纹图谱,其中引物FC8ME10可以把‘闽长茄’与其他茄子品种完全区分开。引物SA1OD3可以把‘敦和茄’和‘苏大青茄’同其他茄子品种区分开来。表明：SRAP标记技术能较好地从分子水平揭示出茄子品种的遗传多样性,并能有效应用于茄子品种指纹图谱构建。
Mostrar más [+] Menos [-]Inglés. Thirty-six eggplant cuhivars were molecularly characterized with SRAP markers. Their genomic DNAs were amplified using 25 primer combinations. A total of 566 bands were generated, of which 102 were polymorphic with an average of 4.08 polymorphic bands per primer pair. The highest genetic similarity was 0. 986 between＇ Wanghaqie＇ and ＇ Benxizaoziqie＇ , while the lowest was between＇ Fushe 1 ＇ and ＇ Huzichangqie＇. A DNA fingerprint of 36 cuhivars was constructed with 8 primer combinations. Primer combination FC8ME10 could distinguish ＇ Minchangqie＇ from the others, and SA1OD3 could distinguish ＇ Dunheqie＇ and ＇ Sudaqingqie＇ from the others. These demonstrated that SRAP was a promising marker system for detecting polymorphisms and identifying unique genotypes in eggplant.
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