Effects of the iron-deficiency on divalent metals in transgenic lines of Malus robusta by introducing LeIRT2 gene | 缺铁胁迫下转番茄铁载体蛋白基因八棱海棠对矿质元素吸收的影响

Chino. 摘　　要：在不同浓度缺铁水培条件下,采用电感耦合等离子体原子发射光谱仪（ICP-AES）测定转基因和非转基因植株叶片和根系Ca2＋、Mg2＋、Ni2＋的含量。研究结果表明：在Fe2＋浓度为1μmol.L-1时,转番茄铁载体蛋白基因（LeIRT2）八棱海棠株系叶片中Mg2＋的含量显著低于非转基因株系,而根系Mg2＋的含量显著高于非转基因株系;该浓度下转基因株系根部Ni2＋含量显著高于非转基因株系,叶片中Ni2＋含量与非转基因株系无显著差异。Fe2＋浓度为10μmol.L-1时,转基因株系叶片和根系Mg2＋、Ca2＋的含量与非转基因株系无显著差异。Fe2＋浓度为1μmol.L-1时,转基因株系叶片和根系Ca2＋的含量与非转基因株系无显著差异。无铁条件下在叶片和根系中Ca2＋、Mg2＋含量无显著差异。

Inglés. The transgenic Malus robusta plants with LeIRT2 were selected for further study.The contents of Ca2＋,Mg2＋and Ni2＋ in leaves and roots of the transgenic lines and non-transgenic plants were detected with the method of ICP-AES.The results showed that with the iron concentration of 1 μmol・L-1,the content of Mg2＋ decreased sharply in leaves but increased notably in roots,compared with the non-transgenic plants.At the same concentration,the content of Ni2＋ in roots was significantly higher than that of the compares,while with no obvious difference in leaves.There were no significant difference of the Mg2＋ and Ca2＋ content in leaves or roots between the transgenic and wild plants treated by iron with concentration of 10 μmol・L-1.There were no significant difference of the Ca2＋ content in leaves or roots between the transgenic and wild plants treated by iron with concentration of 1 μmol・L-1.There were no significance differences of Ca2＋,Mg2＋ content in leaves or roots under iron-free conditions.