SNPs detection of growth hormone gene(GH) in Qingyuan partridge chicken and its genetic effects on growth and reproductive traits | 清远麻鸡生长激素基因(GH)的SNPs检测及其对生长及繁殖性状的遗传效应

Chino. 为了研究生长激素基因(GH)对清远麻鸡部分生长及繁殖性状的遗传效应，基于聚合酶链反应-连接酶检测反应(PCR-LDR)基因多态性并行检测系统，对清远麻鸡(Gallus gallus)生长激素 (GH)基因内含子1的G662A位点和内含子4的T3094C, C3199T位点进行检测，并分析其对部分生长及繁殖性状的遗传效应。检测结果显示，清远麻鸡在3个位点上均产生了3种基因型，LDR分型结果同直接测序结果一致。G662A位点对生长及繁殖性状均不存在显着的效应；T3094C位点上，TT型个体的开产日龄显着晚于CC和CT型个体，其18周龄体重又显著高于CC和CT型个体；C3199T位点上，不同基因型个体仅在50周平均蛋重上存在显着差异，TT型个体的50周平均蛋重显着高于CC和CT型个体。3个位点的合并基因型对开产蛋重、50周平均蛋重以及生长性状均有显着的影响，且对生长性能的遗传效应更加明显，合并基因型AACTCC可以作为筛选清远麻鸡高的体重的分子标记。

Inglés. In order to investigate the association single nucleotide polymorphisms in GH gene with growth and reproductive traits in Qingyuan partridge chicken, the parallel typing system based on PCR-ligase detection reaction (LDG) was established in the present study, and used to detect the polymorphisms of G662A in intron 1 and T3094C and C3199T loci in intron 4 of growth hormone (GH) gene as well as its genetic effects on growth and reproductive traits in Qingyuan partridge chicken (Gallus gallus). The results were as follows: three loci exhibited polymorphisms and were coincidence with direct sequencing. There was no significant effect on growth and reproductive traits at locus G662A; at locus T3094C, individuals with TT genotype had significant older age at first egg and significant higher body weight at the age of 18 weeks than that of CC and CT genotype birds. At locus C3199T, TT genotype birds had significant higher average egg weight at the age of 50 weeks than that of CC and CT genotype birds. The combine genotypes also had significant genetic effects on average egg weight at first laying, average egg weight at the age of 50 weeks, and growth traits. However, it seemed that the effect of GH gene on growth traits was more significant; individuals with AACTCC combine genotype can be used as the molecular genetic marker to select Qingyuan partridge chicken for high body weight.