Effect of different mucolytic agents on viscosity and physical characteristics of dromedary camel semen
2009
El-Bahrawy, K.A. | El-Hassanein, E,E.
6/ml sperm concentration, 64.2 ± 4.3 % mass motility, 23.0± 2.3 % individual motility, 96.6 ± 0.57% intact acrosomes, 5.2 ± 0.4 % primary abnormalities and 8.1 ± 1.7 % secondary abnormalities. After collection, and raw semen analysis each ejaculate was divided into six equal portions and were diluted in a Tris-Lactose extender at 1:3. Each of the five portions was subjected separately to one of the five selected mucolytic agents at a concentration of 50 µl/ml of extender, and the remaining portion was diluted in an extender free from the mucolytic agents (control). Seminal viscosity, sperm motilitly and acrosomal integrity were assessed post-dilution after incubation for 15 min at 37°C and after 240 min of equilibration at 5°C. Amylase superiorly eliminated seminal viscosity in all samples and improved individual sperm motility (46.0 ± 9.12) post-dilution compared to control (27.5± 5.57) and to samples treated with the other mucolytic agents (31.2 ± 5.42 to 37.0 ± 6.55%). However, amylase had a significant (p 0.05) deleterious effect on sperm acrosomal integrity after 240 min of equilibration (11.8± 1.95% detached acrosomes). Although a-chymotrypsin partially liquefied about 60% of samples post-equilibration, it significantly (p 0.05) improved individual sperm motility (46.0 ± 6.35%) and significantly (p 0.05) minimized acrosomal detachment after 240 min of equilibration (7.4± 0.67%). In conclusion, a-amylase totally eliminated viscosity in camel semen, and significantly improved sperm forward motility as compared with untreated semen. However, these mucolytic agents may have deleterious effects on acrosomal integrity after equilibration.
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