Effect of gamma rays on propagation of Zantedeschia aethiopica spreng by tissue culture technique
2011
Arafa, A.M.S. | Hussein, M.M.M. | Nofal, F.H. | Hosny, F.M.
The average of two seasons investigation was initiated at the Laboratory of Plant Tissue Culture, Agricultural Development Systems Project (ADSP), Ministry of Agriculture, to disclose the individual and combined effects of five gamma ray doses (0, 3, 6, 9, 12 and 15 kr) and various growth substances supplementation to MS media, in vitro, on growth and root parameters of Zantedeschia aethiopica (L.) Spreng, shoot tips. The obtained results revealed that survival (%) increased up to the third day, then declined gradually. Survival (%) decreased progressively as irradiation doses (Kr) increased, but plantlets treated with doses higher than 6.0 Kr died at the seventh day. In general, 3.0 Kr irradiation individually at most growth substances, increased the number of shoot, shoots length, number of roots, and root length compared to the control. The response of the studied parameters to various growth substances individually or combined with gamma ray doses was variable. The highest values of shoot length(cm) and the number of shoots resulted significantly by 3.0 Kr irradiation combined with 4.0mg BA/l and 3.0 Kr irradiation combined with 3.0mg TDZ/1, respectively in comparison to most other treatments. Moreover, the number of roots and root length were significantly the largest due to 3.0 Kr gamma rays with 5mg NAA/1, successively compared with most other treatments. Whereas, the lowest values of shoot length(cm) and the number of shoots were produced by 6.0 kr gamma rays combined with 0.0mg BA/L and 0.0mg TDZ/1, on concession.
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