Artificial Spawning and Fingerling Culturing of European Catfish (Silurus glanis L. 1758)

The present study was conducted to survey the artificial spawning of European catfish (Silurus glanis L.) by synthetic hormones include Carp pituitary extract (CPE), Ovofact [D-Ala6 des _ Gly10] GnRHa Ethyl amid in combination with dompridone diluted in propylene glycol, Human gonadothropin chryonic hormone (HCG) and LHRHa (des-Gly10, [D-Ala6] LH-RH Ethyl amide). In this research work 44 male and 72 female brood stokes that transferred to the Shaheed Dr Ansari hatchery and Sefid rod research station were studied on 2 years and injected with those stimulated maturation. Total length, total weight, gonad weight were measured to the nearest 0.1 cm, 1g and 0.01g respectively. The experiments were conducted using different doses of hormones on 4 treatments.In treatment CPE that brood stocks injected with 2.5, 3, 4 and 5 mg/kg/BW, the most responded (83.33%) was with 5mg/kg/Bw of CPE. The mean fertilization in this treatment was 84 92.5±4.51 and after that there are groups which were stimulated by 4 mg/kg/BW of CPE .In treatment ovafact that brood fish were injected by 10, 20, 30, 40 µg/kg/BW of this stimulation hormone showed 100 percent of fish ovulated in the group treated with 40 µg/kg/BW of ovofact. The mean fertilization rate was 86.08 ±6.27 in this group. 33.3 % of brood fish ovulated in the group that injected with 40 µg/kg/BW of LHRHa stimulation, but none of brood stock reached to spawning with injection of 30 µg/kg/BW of LHRHa. The brood stocks were injected by 800, 850, 900, 950 IU/kg/BW of HCG, only 33.3 percent of fish in groups that received 900 & 950 IU/kg/BW of this Stimulation hormone reached to spawning stages.According to our results and statistical analysis between trial treatments in percent of Embryo viability showed significant difference (P0.05) between HCG treatment with Ovofact and CPE groups. This results indicates that embryo viability was better in CPE and Ovofact rate to LHRHa and HCG treatments. In the other cases include the working fecundity, fertilization percent, latency period of maturation, latency period of incubation eggs and ratio of weigh stripped egg to body weight did not show any significant difference (P0.05) in experimental treatments for stripping the male brood stock of European catfish were injected by 3,4,5 mg/kg/BW of CPE, but not one of male brood fish didn't reaches to stripping stage , so for eggs fertilization were used of milt from macerated Testes of dead males. So in anther experiment the male brood fish were injected by 5 mg/kg/BW CPE repeatedly at once every 5 days for Period 20 days period and milt were collected from of all of the males.