Effect of carbohydrate, gibberellic acid, indolebutyric acid, phloroglucinol, explant orientation and culture vessels volume on optimizing in vitro propagation of M.9 apple rootstock
2003
Miri, S. M | Vaez Livari, B, Iranian Research Organization for Science and Technology | Khalighi, A | Ghaem maghami, S. A, Iranian Research Organization for Science and Technology
gibberellic acid (GA3), indolebutyric acid (IBA), phloroglucinol (PG), explant orientation and culture vessels volume were studied. The basal medium was MS supplemented with 1.5 mg.1-1 BA and 1 mg.1-1 Kin. Shoot explants 1 cm in length were taken from in vitro samples. The results showed that sorbitol was more suitable as a carbohydrate for multiplication than sucrose. GA3 and PG had no effect on number of shoots, but affected length of shoots. Application of IBA had no effect on proliferation. Explant orientation has significant effect on proliferation and the best resutls were obtained with vertical orientation. Increase in vessel volume from 25 ml to 125 ml had no effect on shoots proliferation of M.9. On the basis of the results, is suggested for improving of M.9 proliferation were used from basal medium culture (MS with 1.5 mg.l-1 BA, 1 mg.l-1 Kin and 8 g.l-1 agar) containing 30 g.l-1 sorbitol, 0.1 mg.l-1 GA3, 162 mg.l-1 PG. Also, shoots put in meduim vertically.
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