Application of Microsatellite Markers for Identification and Registration of Date Palm Cultivars
2010
Mardi, Mohsen | Torahi, A | Kavand, A.R.
Due to complex identification of young date palm cultivars (Phoenix dactylifera L.) using morphological traits, advance molecular tools have provided a new prospect for DNA fingerprinting. In this study, specific molecular keys were identified for 10 Iranian date palm cultivars (Phoenix dactylifera L.) using 7 SSR markers. The results showed that 7 SSR markers produced polymorphic for studied Iranian date palm cultivars. SSR markers MPA 4 in Estameran, MPA 6 and 10 IN Piarom, MPA 10 and 12 in Shahani and Piarom produced specific molecular keys. Furthermore, combination marker MPA 6-12 in Kabkab, Mazafati and Shahani, MPA 5-10 In Berhi and Shahani, MPA 10-12-6 in Gantar and MPA 16-6-12-4- 7-10 in Deiri produced specific keys. The results showed that there was a different genetic background within Deiri, Rabi and Piarom mother's trees. The specific molecular keys were verified on 50 date palm mother's trees and the results were confirmed at two independent laboratories. The reported specific molecular keys can be used for identification of 10 Iranian date palm cultivars.
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