AFLP fragments linked to fruit skin colour in the oil palm (Elaeis guineensis Jacq.)
2007
Seng, T.Y. | Faridah Qamaruz Zaman, Putra Malaysia Univ. (UPM), 43400 UPM Serdang, Selangor (Malaysia). Inst. of Bioscience | Ho, C.L., Putra Malaysia Univ. (UPM), 43400 UPM Serdang, Selangor (Malaysia). Faculty of Biotechnology and Biomolecular Sciences | Maizura Ithnin, Malaysian Palm Oil Board, No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor (Malaysia) | Vengeta Rao, Boh Plantations Sdn. Bhd.
The oil palm (Elaels guineensis ]acq.) has two fruit colour forms: nigrescens (Nig), unripe fruits deep violet ripening to reddish orange and virescens (Vir), immature fruits green ripening to bright orange. Vir is monofactorially dominant over Nig. Fruit colour change during ripening is more obvious in Vir, and could be exploited as a harvesting criteria, as the present practice of waiting for abscinding fruits requires considerable labour to collect the fruits. Molecular markers would be very useful in Vir breeding as oil palms take about three years to bear fruits. A preliminary screening of 64 commercial AFLP primers showed 10 to be informative for the two populations studied (a CBP and a NPC cross from a Pamol Plantations virescens breeding programme). Three fragments were found to closely tag the trait in a BSA sample of 10 individuals for each colour type from each population. When tested on the full populations, two E-ACT/M-CAT fragment s, 142.13 bp and 355.76 bp long respectively distinguished Vir with 83% accuracy in CBP and NPC while a 253.79bp fragment from E-ACT/M-CTA distinguished Nig with 77% accuracy in both populations.
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