Karcinogen nitrozaminok a dohanyban es a cigarettafustben.
1992
Makleit S. | Pocsi K. | Vass S.
The samples used in the experiment consisted of 25.0 g finely powdered tobacco and 50 cigarettes. The latter were smoked mechanically. Extraction was carried out for 24 h using ascorbic acid citrate buffer with a pH of 4.5, after which the solution was filtered and shaken with ethyl acetate. The ethyl acetate phase was evaporated on a rotational vacuum evaporator and purified on an Al203 column. The eluate was evaporated to dryness and the NNN concentration of the standard material (NNN) was determined in methanol solution using UV spectrophotometry. The material used in the experiment was 74.5 o/o as regards NNN. The NNN peak of Burley type tobacco was considerably higher than that of Virginia type tobacco, while the NO3-N content of naturally dried, large-leaved tobaccos was 10 times greater and the NNN content twice as great as in the Virginia type. The best NNN-reducing material proved to be K ascorbate, which inhibits NO2 formation (25-35 o/o), vitamin E antioxidant only reduced the NNN content by 20 o/o.
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