Changes of plasma progesterone concentrations after induction of estrus in the bitch.
1989
Kang B.K. | Choi H.S. | Lee C.B. | Oh K.S. | Son C.H. | Na J.S.
This study was performed to investigate the patterns of progesterone secretion after induction of estrus in premature, metestrous and anestrous bitches. A total of 22 bitches were used. 18 bitches were treated with hormone to induced estrus and 4 bitches were untreated and served as controls. Estrus was induced with PGF 2 alpha, estrone, estradiol-17 beta, PMSG and HCG (Treatment A), and with PMSG and HCG (Treatment B). Blood samples were collected via the cephalic vein at 2 to 5 days interval. Blood samples were centrifuged (1,200g, 10min.) within 30 minutes after collection and plasma was stored at -20deg C until analyzed for the progesterone concentrations. Plamsa progesterone concentrations were measured by radioimmunoassay. The results of estrous induction were determined by estrous signs, overain response, egg recovery and progesterone patterns. All bitches in treatment A showed estrous signs, however the ovarian response and egg recovery were not detectable and the levels of progesterone were nearly same as before. In the treatment B, premature and metestrous bitches showed only estrous signs, however 5 of 7 anestrous bitches (71.4 %) showed estrous signs, ovarian response and changes of progesterone levels. In conclusion, clinical estrous behavior can be induced during any phase of the estrous cycle, but ovulation should be induced only if induction occur approximately 4 months or more after the previous estrus.
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