Evaluation of the potency test method for Akabane disease live-virus vaccine using hamster.
1995
Shimazaki T. | Nakamura S. | Inoue Y.
Presently, the potency test of Akabane disease live-virus vaccine has been evaluated using cattle which are the target animals of Akabane virus. Presiously, we reported that Syrian hamster exhibited a high neutralizing antibody response when inoculated with the vaccine by the intraperitoneal (ip) route. In this report, we examined the potency test method for Akabane disease live-virus vaccine using hamster instead of cattle. The difference in the age o9f the hamsters did not affect the neutralizing antibody titer and similar titer was recorded in 4, 5, 6 and 8-week-old hamsters. The geometric means (GM) of neutralizing antibody titers of hamster inoculated with vaccines from 4 manufacturers (6 lots) ranged between 445.7 and 2352.5. These results indicated that there was no significant difference among the GM values in the experiments. In the hamsters inoculated by the ip route with the vaccine (1 dose), the vaccine virus was recovered from the lung, liver and spleen from 12 hours to 1 day after inoculation. Although there was no appreciable difference between hamster, Syrian hamster and GN or CAK (inbreeding strain), there was a significant difference (p=0.01) between GN and CAK. It was shown that the hamsters used in these examinations displayed a stable sensibility to Akabane disease live-virus vaccine and could be used for the potency test of the vaccine. However, it is necessary to carry out further studies because hamsters inoculated with the vaccine which showed a negative response to the potency test currently used produced a high titer of neutralizing antibodies.
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