Plant regeneration from indica and japonica rice protoplasts and cytological and field studies of regenerated plants.
1993
Zapata F.J. | Ghosh G.C. | Wu C.Y. | Zhang S. | Kumar V.M. | Torrizo L.B. | Abrigo E.M. | Alejar M.S. | Chen W.
One of the most significant developments in plant tissue culture has been the isolation and culture of protoplasts. Protoplasts are ideal for gene transfer and generation of variation. Protocols for protoplast isolation from suspension cultures and direct isolation from primary calli, culture and plant regeneration have been established. More than 5000 plants have been regenerated from different varieties of indica, japonica and wild rice. The chromosome behavior and number of 92 IR43 and 35 IR57311-95-2-3 protoplast-derived Ro plants were determined. Sixty-two and 97 percent, respectively of the IR43 and IR57311-95-2-3 regenerants were normal diploids. Tetraploidy and abnormal chromosomal behavior were observed in both cultivars. Taipei 177 protoclones and the seed-derived control were evaluated and compared on thirteen agronomic traits, grain quality characteristics and yield components under field conditions for two seasons. Significant variations both positive (+) and negative (-) relative to control were observed for number of days to flower (-), culm length (-), panicle length (+), flagleaf length (+), flagleaf L-W ratio (+) and primary branch per panicle (+) in the protoplast-derived lines. No significant difference in yield was observed.
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