Simple technique for mass propagation of Beuaveria bassiana for microbial control in Thailand
2007
Saengyot, S, Mae Jo Univ., San Sai, Chiang Mai 50290 (Thailand). Upper Northern Regional Center, National Biological Control Research Center | Napompeth, B., Kasetsart Univ., Bangkok (Thailand). National Biological Control Research Center
A number of media for culturing and mass propagation of the enthomopathogenic fungus, Bueaveria bassiana strain NBCRSO1 were screened under laboratory condition. At 25-30 deg C the artificial media, Sabouraud Dextrose Agar (SDA) and Malt Extract Agar(MEA) were the most suitable ones.They promoted highest mycelium growth rate and sporulation averaging 0.33 +- 1.24 cm per day and 7.28 +- 1.07 x10sup8 spores per ml respectively.Likewise steamed sorghum seed was the most suitable medium for its mass propagation. It could promote within a 14 day period averaging 100%, 99.33 +- 1.54% and 1.2 +- 1.1 x 10sup10 spores/ml respectively for hyphal colonization, proportion of sporulation area on the media, and spore concentration.The results from this preliminary study could be further applied for the utilization of B. bassiana as a microbial control of several insect pest species adopting this simple method for mass propagation by the small farmers.
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