Seed coating with mixed bacterial antagonist and sticker agent for control of Aspergillus flavus infection of sweet corn

Kasem, S.; Prathuangwong, S., Kasetsart Univ., 10900 . Dept. of Plant Pathology; Juthawantana, P., National Corn and Sorghum Research Center, Nakhonrachasrima; Ngamprasitthi, S., Kasetsart Univ., Bangkok 10900 . Inseechandrastitya Inst. for Crop Research and Development

Seed coating with mixed bacterial antagonist and sticker agent for control of Aspergillus flavus infection of sweet corn

2009

Kasem, S. | Prathuangwong, S., Kasetsart Univ., 10900 (Thailand). Dept. of Plant Pathology | Juthawantana, P., National Corn and Sorghum Research Center, Nakhonrachasrima (Thailand) | Ngamprasitthi, S., Kasetsart Univ., Bangkok 10900 (Thailand). Inseechandrastitya Inst. for Crop Research and Development

The experiment was conducted in laboratory and greenhouse conditions to determine the effect of sweet corn seed coating technique using various sticker agents combined with 3-antagonistic bacterial strains, Bacillus amyloliquefaciens KPS46, Pseudomonas fluorescens SP007s and Serratia marcescens Spt360 under different storage conditions. The percentage of seed germination and seedling vigor of sweet corn cv. insee2 was evaluated at 15-day interval for 3 months using blotting and pot bioasssay. Coated seed treatment with different sticker agents including carboxy methyl cellulose, Tween80 and TensionT7 exhibited lower percentage of seed germination and seedling vigor (shoot and root length) than antagonistic bacteria coated seeds, except polyacrylate (PLA) and chitosan extract that showed highest percentage seed germination but not seedling vigor. To improve the efficacy of seed coating against Aspergillus flavus infection, seeds coated with each antagonistic bacteria PLA and chitosan combinations were assessed. PLA and chitosan combined with antagonist were better effective than antagonist alone. Seed coated with PLA + SPOO7S or chitosan + SPOO7S were significantly highest (P0.05) in enhanced seed germination and seedling vigor, where seed coated with PLA + KPS46 significantly reduced Aspergillus flavus incidence. None of these sticker agents showed negative effect on seed health and survival of benefit bacteria on seeds that indicated the advantage of improved efficacy of antagonism and PGPR. However, the storage temperature was important factor affecting survival and efficacy of antagonistic bacteria coated onto the seeds. Coated seed stored at room temperature showed a 2-4 fold decrease in bacterial density compared to coated seed stored at 10 deg C.

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