Application of ion chromatography coupled with pulse amperometric detection to determine myo-inositol content in plant components of feed
2011
Dulinski, R. | Stazynska-Janiszewska, A.,Uniwersytet Rolniczy, Krakow (Poland). Katedra Biotechnologii Zywnosci
In the research project, an ion chromatography technique was used as an alternative to microbiological assay to determine the content of myo-inositol in plant components of feed. The analyzed material consisted of both genetically non-modified and modified varieties of wheat, corn, and soybean, as well as of feed mixtures made on the basis thereof. The contents of total and free inositol were determined as was the content of dialyzable inositol released by in vitro procedure to simulate the intestinal tract of broilers. The content of total inositol determined by the HPAEC-PAD method ranged between 2572 micro g/g (corn-soybean feed) and 3667 micro g/g (corn). The data obtained were comparable only with the results of the microbiological assay of both the genetically non-modified and modified soybean pellets. As regards the other feed components, the results obtained by the HPAEC-PAD method were higher than those obtained using the microbiological method, on average: by 30 to 50 percent
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