Silencing of two tomato prolyl 4-hydrosylases induces alteration in growth an[d] fruit ripening programs
2009
Samir Zaki, M.
Prolyl 4 hydroxylases are 2-oxoglutarate dependent dioxygenases which hydroxylate proline-rich peptides, a major post-translational modification of cell wall proteins. In the tomato genome, 9 putative P4Hs have been identified up to now clustered into 3 different subgroups according to their polypeptide sequence. Two of them, SlP4H5 and SlP4H7, were selected in order to investigate their physiological significance in plant growth and development using gene silencing approaches such as VIGS (virus induced gene silencing) and RNA interference. Transient silencing with VIGS resulted in young tomato plants with decreased root:shoot biomass ratio which might be attributed to changes in hormonal balance between auxin and cytokinin. In addition, transient silencing of attached to the vines tomato fruits caused irregular ripening progression monitored through colour development of fruits. These preliminary results indicate involvement of P4Hs in plant growth and development.
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