Analysis of resistance to Yam mosaic virus, genus Potyvirus in white Guinea yam (Dioscorea rotundata Poir.) genotypes
2011
Babajide, O.O., Obafemi Awolowo University, Ile Ife, Osun State (Nigeria). Faculty of Agriculture | Asiedu, R., L.W. Lambourn and Co., Croydon (United Kingdom). International Institute of Tropical Agriculture | Shoyinka, S.A., Institute of Agricultural Research and Training, Ibadan, Oyo State (Nigeria) | Hughes, J. d’A., The World Vegetable Center, Shanhua (Taiwan). Asian Vegetable Research and Development Centre
Resistance to Yam mosaic virus (YMV), genus Potyvirus was studied in 10 populations of selected white Guinea yam (Dioscorea rotundata). Plants of resistant genotypes: TDr 35, TDr 1621, TDr 93-1, TDr 93-32, TDr 95- 107, TDr 93-23, and susceptible ones: TDr 87/00211, TDr 87/00571 and TDr 95-127 were screened for their reaction to the pathogen by symptom severity scoring scale of 1-5, and by quantifying virus multiplication by triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA). Controlled crosses were made among the genotypes within and between the groups according to reactions to the pathogen. The resultant F1 progenies were evaluated for the infection by disease symptom development and by TAS ELISA to detect a symptomless infection in an insect-proof screenhouse for the assessment of inheritance of resistance to YMV. A genetic analysis of the reactions of progenies derived from the D. rotundata genotypes to inoculation with YMV strongly suggests that resistance to the virus is a dominantly inherited trait. Segregation ratios obtained from the families indicate that at least two dominant genes are involved.
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