Production of Bacillus thuringiensis S6 biomass using different cheap nitrogen sources
2011
Dhingra, H., Mody Institute of Technology and Science, Rajasthan (India). Faculty of Arts, Science and Commerce, Department of Science | Chaudhary, K., CCS Haryana Agricultural University, Hisar (India). Department of Biotechnology and Molecular Biology
In recent years, Bacillus thuringiensis is receiving increasing attention for its use in integrated pest management programs for agricultural and forest insect pests and insect vectors of human and other mammalian transmissible diseases. As the overuse and negative impacts of chemical pesticides on soil, water, human health, wildlife and ecological balance have led to the use of microbial insecticides against insect pests of order Lepidoptera, Coleoptera, Diptera and Hemiptera, an attempt has been made to develop low cost Bt formulations for the management of Helicoverpa armigera. Agroindustrial residues and their byproducts can be used for large scale production of B. thuringiensis biomass. Different low cost agro-industrial based nitrogen sources such as cotton seed meal, soya meal, sunflower meal, urea, ammonium sulfate and potassium nitrate were evaluated for Bt biomass production. Among the different nitrogen sources evaluated, maximum biomass of B. thuringiensis isolates was achieved in the medium containing 1.0% cotton seed meal in basal medium. The biomass production in this medium was equivalent to that achieved in LB medium after 48h of growth.
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