Comparative studies of isolates of Colletotrichum spp. the causal agents of anthracnose. [Doctoral dissertation] | Uporedna proučavanja izolata Colletotrichum spp. prouzrokovača antraknoze. Doktorska disertacija
2011
Živković, S.
In Serbia, the occurrence of anthracnose on pear, apple, sour cherry, and tomato fruits has been recorded during the last several years. Typical fruit symptoms include dark, sunken, and circular lesion with orange conidial masses. Colletotrichum spp. was isolated from all diseased fruits. Twenty monoconidial isolates were selected for further comparative studies. Identification was carried out based on the conventional, morphological, physiological and pathogenic features, as well as molecular characterization of tested isolates. The reference strains of C. acutatum (CBS 294.67) and C. gleosporoides (CBS 516.97) were used as a control. Patogenicity tests with representative isolates were conducted on symptomless detached fruits. All tested isolates caused anthracnose lesions of pear, apple sour cherry, and tomato fruits after 7 days of incubation. Koch's postulates were fulfilled by reisolation from inoculated fruits. All examined characteristics of the isolates were analyzed as possible taxonomic criteria for differentiation among Colletotrichum species. Because of outstanding variability, most of the studied characteristics cannot be independently applied as taxonomic criteria. Obtained results showed that, for Colletotrichum species classification and proper disease diagnosis, at least two taxonomic criteria should be used: morphological features combined with molecular identification.
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