Expression analysis of boiling-stable protein (BsCyp) in response to drought, salt and osmotic treatments in drought tolerant and susceptible cultivars of Triticum aestivum
2011
Rakhra Gurmeen | Sharma Arun Dev | Singh Jatinder | Vyas Dhiraj
The effect of osmotic, drought and salt stresses on the induction of boiling-stable proteins were studied in C-306 (drought tolerant) and HD-2004 (drought susceptible) cultivars of Triticum aestivum. Western blotting analysis, using polyclonal antibodies raised against a 20 kDa A. thaliana cyclophilin, revealed the induction of a cross-reacting band (40 kDa, BsCyp) in cv. 306. However no substantial expression was observed in drought sensitive cv. HD-2004, indicating its role in water stress adaptation. Thermostability studies of BsCyp in cv. 306 revealed that BsCyp was stable and can be detected even after 20 min of boiling treatment under all the stresses. Based upon these observations the possible role of BsCyp in water stress tolerance is discussed.
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