HRM analysis of beta-lactoglobuline gene in Slovak Pinzgau breed | HRM analýza beta-laktoglobulínového génu slovenského pinzgauského plemena
2010
Miluchová, M., Slovenská poľnohospodárska univerzita v Nitre (Slovak Republic), Fakulta agrobiológie a potravinových zdrojov, Katedra genetiky a plemenárskej biológie | Trakovická, A., Slovenská poľnohospodárska univerzita v Nitre (Slovak Republic) | Kadlečík, O., Slovenská poľnohospodárska univerzita v Nitre (Slovak Republic) | Kasarda, R., Slovenská poľnohospodárska univerzita v Nitre (Slovak Republic) | Hazuchová, E., Slovenská poľnohospodárska univerzita v Nitre (Slovak Republic)
The goal of the paper is to identify the beta-lactoglobulin gene polymorphism and to analyse the genotype structure of Pinzgau cattle population. The beta-lactoglobulin (LGB) is synthesized in milk and is important for evaluation of milk production potential and butterfat and protein content. LGB is localized on bovine chromosome 11. The AAgenotype of LGB is associated with higher milkyield, the BB genotype with higher fat and casein content and is more desirable for cheese making. The sample involved 39 cattle animals. Bovine genomic DNA was isolated by fenol-chlorophorm deproteinization and ethanol precipitation and used in order to estimate LGB genotypes by means of PCR RFLP method and HRMA. There homozygote genotype AA with frequency 7.69%, heterozygote genotype AB with frequency 25.64% and homozygote genotype BB with frequency 66.67% were detected in evaluated Pinzgau population. The results point out that the frequency of B allele was very high and reached 79.49% of the population. The frequency of A allele reached 20.51%.
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