The effect of rapamycin on secretory activity of the rabbit ovarian fragments
2012
Kádasi, A., Slovak Univ. of Agriculture in Nitra (Slovak Republic). Faculty of Biotechnology and Food Sciences, Department of Animal Physiology | Sirotkin, A. V., Animal Production Research Centre, Nitra (Slovak Republic) | Sushmita Nath, Assam University, Silchar (India). Department of Life Science and Bioinformatics | Kolesárová, A., Slovak Univ. of Agriculture in Nitra (Slovak Republic) | Maruniaková, N.
The aim of our study was to examine the effect of rapamycin on secretory activity of the rabbit ovarian fragments. The secretion of steroid (progesterone, testosterone, estradiol) and peptide (prolactine) hormones by ovarian fragments after rapamycin addition at the doses 0, 1, 10, 100 micro g per ml was determined. Fragments were incubated with rapamycin for 48 hours. Hormones were determinated by RIA. The experimental data showed that, addition of rapamycin did not affect progesterone and prolactine release (at all doses). Estradiol secretion was inhibited by rapamycin at the doses of 1, 10 and 100 micro g per ml. Testosterone was inhibited by the rapamycin at the doses of 1 and 10 micro g per ml but not at 100 micro g per ml. In conclusion, our results suggest a direct effect of rapamycin on ovarian functionsand a possible involvement in the regulation of steroidogenesis.
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