“绿岭”核桃种胚组培苗腋芽快繁体系的初步研究 | Rapid Propagation of “Lv Ling” Walnut (Juglans Regia L.) through Embryo-Induced Seedlings

Chino. <p> <span style="font-family:宋体;font-size:10pt;">以早实薄皮核桃</span><span style="font-family:宋体;font-size:10pt;">“</span><span style="font-family:宋体;font-size:10pt;">绿岭</span><span style="font-family:宋体;font-size:10pt;">”</span><span style="font-family:宋体;font-size:10pt;">成熟种胚诱导出的腋芽为外植体，</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">MS</span><span style="font-family:宋体;font-size:10pt;">为基本培养基，通过调节激素种类和浓度配比，初步研究了</span><span style="font-family:宋体;font-size:10pt;">“绿岭”</span><span style="font-family:宋体;font-size:10pt;">核桃种胚组培苗腋芽快繁体系。比较试验结果表明：最佳种胚萌发培养基为</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">MS + </span><span style="font-family:宋体;font-size:10pt;">蔗糖</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">30 g/L + </span><span style="font-family:宋体;font-size:10pt;">琼脂</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">6 g/L</span><span style="font-family:宋体;font-size:10pt;">；最佳腋芽诱导和增殖培养基为</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">MS + 6-BA 2.0mg/L + NAA 0.2 mg/L + </span><span style="font-family:宋体;font-size:10pt;">蔗糖</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">30 g/L + </span><span style="font-family:宋体;font-size:10pt;">琼脂</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">6 g/L</span><span style="font-family:宋体;font-size:10pt;">。生根采用两步生根法：先在</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">DKW + IBA 8.0 mg/L + </span><span style="font-family:宋体;font-size:10pt;">蔗糖</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">30 g/L + </span><span style="font-family:宋体;font-size:10pt;">琼脂</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">6.0 g/L</span><span style="font-family:宋体;font-size:10pt;">暗培养</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">20 d</span><span style="font-family:宋体;font-size:10pt;">，然后转入</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">DKW + </span><span style="font-family:宋体;font-size:10pt;">蔗糖</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">30 g/L + </span><span style="font-family:宋体;font-size:10pt;">琼脂</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">6 g/L</span><span style="font-family:宋体;font-size:10pt;">培养基中进行光照培养，生根率为</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">50%</span><span style="font-family:宋体;font-size:10pt;">。诱导生根的组培苗移栽到大田后成活率为</span><span style="font-family:'Times New Roman','serif';font-size:10pt;">33.3%</span><span style="font-family:宋体;font-size:10pt;">。

Inglés. </span> </p> <span style="font-size:10pt;">The mature embryo induced axillary buds were used as explants to investigate rapid propagation of precocious thin-shelled “Lv Ling” walnut in the MS basic medium with different concentrations of plant growth regulators. The results showed that MS + sucrose 30 g/L + agar6 g/L was the most feasible medium for embryo germination, and MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L + sucrose30 g/L + agar6 g/L was the best for induction and multiplication of the axillary buds in all tested treatments. The 50% of rooting rate was achieved by a two-stage rooting method, namely, the shoots were first cultivated in DKW + IBA 8.0 mg/L + sucrose30 g/L + agar6.0 g/L medium for 20 days under dark condition, then transferred to DKW+ sucrose30 g/L + agar6.0 g/L medium under light condition. After transplanting to the field, 33.3% of the seedlings survived.<span></span></span> <p> <span style="font-family:宋体;font-size:10pt;"></span>  </p>