TRANSCRIPTIONAL ACTIVITY OF TRANSLOCATED NORs IN BARLEY - HORDEUM VULGARE L.
2012
Kitanova, M., Sofia University 'St. Kliment Okhridski', Sofia (Bulgaria) | Georgiev, S., Sofia University 'St. Kliment Okhridski', Sofia (Bulgaria)
The standard barley cultivar Freya and structural mutant forms ab, s, q, T21 and T627 were used to investigate the transcriptional activity of homologous rDNA loci - NORs based on the positive reactions of NORs of metaphase chromosome with AgNO3 and the number and size of silver stained nucleoli in somatic interphase cells and meiocytes. Fluorescent in situ hybridization - FISH was also used. The chromosomal rearrangement enables testing of intrachromosomal suppression of NORs and provides insight into the mechanisms of intraspecific nucleolar dominance. Considerable difference in gene expression - nucleolar dominance after AgNO3 and FISH was established only in line ab, when both NORs - 5H and 6H rDNA loci are co-localized on the same or opposite arms of the chromosome. Translocation-induced intraspecific nucleolar dominance is probably the result of interaction of NOR6H and NOR5H or other genetic factors like up-elements located on the NOR-bearing chromosomes. The significance of such an unusual phenomenon is discussed.
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